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Anti-Bacterial Activities of Crude Cell Free Supernants of Lactic Acid Bacteria from Wara (Nigerian Soft Cheese)

Ibrahim TA and Falegan CR

Crude cell free supernant (CFSs) of lactic acid bacteria from wara were isolated and tested for their invitro antibacterial activities against selected clinical pathogenic bacteria such as Salmonella sp, Staphylococcus sp, Bacillus sp, E.coli and Klebsiella sp. Wara samples were collected from hawkers in sterile container and grown on the surface of nutrient agar and MRS agar at 370C for 24 hours. The CFSs were obtained by centrifugation. The antibacterial activity of the CFS was carried out using agar well diffusion assay. The zones of inhibitions were 0.6±0.1, 1.5±0.03, 0.0±0.0, 0.0±0.0 and 0.8±0.0 respectively. Staphylococcus aureus was the most susceptible with the diameter of inhibition zone range between 1.5±0.03mm followed by Klebsiella sp 0.8±0.0mm/ml, Salmonella sp 0.6±0.1mm. CFSs showed no activity against E-coli and Bacillus sp. These data suggest that the CFSs could be used as a good source of nutraceutical.