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Isolation and Antimicrobial Evaluation of Bixa orellana and Linum usitatissimum

Mital Patani, Mandev Patel, Khomendra Sarwa and Anil Bhadari

This research work was carried out to investigate the antimicrobial activities of isolated fraction of Bixa orellana and Linum usitatissimum against five bacterial and five fungal pathogen. Leaves Powder of bixa orellana and seed powder of Linum usitatissimum subjected to soxhlet extraction. The separation of various fraction was carried out by column chromatography, analyzed and purified by thin layer chromatography(TLC) until homogeneity of the fraction achieved. The homogeneity and the purity of the different fractions were checked by HPLC. Antimicrobial evaluation of isolated fractions was carried out. Two fraction Fr-1 (352mg) and Fr-2(260mg) were collected from Bixa orellana had Rf was o.99 and 0.35. fraction 3 and 4 was isolated from linum usitatissimum with Rf 0.45 and 0.25. When compare to the standard drug gentamycin, phenolic fraction of 1 and 2 had good antibacterial activity against all bacterial strain where as glycoside fraction 3 and 4 were poorest activity against all bacterial strain. When compare to standard drug tioconazole fraction-1 and 2 had very good activity against fungal strain and fraction 3 and 4 had moderate activity against fungal strain.