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Comparative Study of Effect of Different Tissue Culture Media on the Propagation of Potato

Mona Rani, Shekhar Kumar, Sumita Shukla, Mradula Sachan and Biresh Sharkar

Tissue culture study on potato was investigated in this paper. MS basal medium supplement with 30 and 40 g/l sucrose, 8 g/l agar and different plant growth regulator were used. Potato was cultured in different ratio of IBA: BAP (10:10, 10:5, 5:10, 5:5 μM) in combination. Development was not identical in all the treatments, cultured tissues were observed in treatments about a week after culture. Results showed that the treatments containing 10 μM IBA in combination with 10 μM BAP is suitable for callus induction. While the tissue formed in 5:5 μM IBA: BAP was found to be delicate, short. Also, the tissue formed in 5:10 IBA: BAP was delicate, short but was higher in density as compared to treatment contained 5:5 μM IBA: BAP.