Abstrato

Development and Assessment of Antioxidant, Anticancer Activity of Poly-Herbal Combination

Binay Kumar Mahto, Ajay Kumar Shukla

The occurrence of lung cancer and related death is increasing yearly. It is the leading cause of cancer death amongst women worldwide. Hence, there is a need to find the alternative cure for the treatment and management of lung cancer with minimal side effects. The present research work objective was development of the poly-herbal capsule formulation comprising of the hydro-alcoholic (20:80) extracts using Ocimum sanctum, Curcuma longa, Emblica officinalis, Teriminalia bellerica, Teriminalia chebula, Piper longum, Piper nigrum and Zingiber officinale for the management of lung cancer disease. This poly-herbal capsule formulation is having potential antioxidant and anticancer bioactive molecule. The poly-herbal capsule formulation was formulated and standardized as per WHO guidelines of quality standardization. The optimized poly-herbal capsule formulation was evaluated by using standard evaluation parameters of capsule formulation. The antioxidant and anticancer activity was determined by using DPPH (2, 2-diphenyl-1-picrylhydrazyl) and ferric ion reducing power assay (FRAC) and (MTT) 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide on A549 lung cancer cell line. The evaluation results of pharmaceutical parameters i.e. weight variation; and moisture analysis was found to be within official limit. The dissolution studies represented that drug release of maximum up to 98.67% at 30 minutes. The DPPH assay and FRAC assay activity of the poly-herbal extract is found to potent as similar standard drug. The optimized finished poly-herbal formulation showed significant cytotoxic activity against A549 lung cancer cell line. The results of all emulation parameters revealed that it could be used for the treatment and management of lung cancer disease.